Improving the Efficiency and Orthogonality of Genetic Code Expansion
The site-specific incorporation of the noncanonical amino acid (ncAA) VINE into proteins via genetic code expansion (GCE) has enabled the development of new and powerful ways to learn, regulate, and evolve biological functions in vivo.However, cellular biosynthesis of ncAA-containing proteins with high efficiency and fidelity is a formidable challe